Investigations into Isoniazid Treated Mycobacterium tuberculosis by Electrospray Mass Spectrometry Reveals New Insights into Its Lipid Composition

Table 1

Molecular formula of the lipids corresponding to observed m/z values, obtained from Mtb LipidDB (MS-LAMP).

(a)

Control (Untreated)
S. No.	Observed m/z [M+H]^+	Fatty Acyls (FAs)	Glycerolipids (GLs)	Glycerophospholipids (GPLs)
1	317	-	C18 H36 O4	-
2	331	-	C19 H38 O4	-
3	409	C27 H52 O2	-	-
4	485	-	C30 H60 O4	C22 H45 O9 P1
5	497	-	-	C23 H45 O9 P1
6	513	-	C31 H60 O5	C24 H49 O9 P1
7	539	-	C33 H62 O5	-
8	611	-	-	C28 H51 O12 P1
9	626	-	C39 H76 O5	-
10	650	-	C41 H76 O5	-
11	694	C46 H92 O3	C44 H84 O5	-
12	706	C48 H96 O2	C45 H84 O5	-
13	872	-	C56 H102 O6	-
14	1054	-	C69 H128 O6	-
15	1240	C83 H162 O5, C84 H166 O4	C82 H158 O6	C65 H123 O19 P1

(b)

INH (Treated)
S. No.	Observed m/z [M+H]^+	Fatty Acyls (FAs)	Glycerolipids (GLs)	Glycerophospholipids (GPLs)
1	343	-	C20 H38 O4	-
2	369	C24 H48 O2; (2 nos.)	-	-
3	411	C27 H54 O2	-	-
4	413	C26 H52 O3	C25 H48 O4	-
5	425	C28 H56 O2	-	-
6	467	C31 H62 O2 (2 nos.)	-	-
7	483	C31 H62 O3	-	C22 H43 O9 P1
8	496	C33 H66 O2 (2 nos.)	-	C24 H50 N1 O7 P1
9	511	-	-	C24 H47 O9 P1
10	512	C33 H66 O3	-	-
11	551	-	C34 H62 O5	-
12	624	-	C39 H74 O5	-
13	700	-	-	-
14	734	-	C47 H88 O5	C39 H73 O10 P1
15	738	-	C46 H88 O6, C47 H92 O5	C39 H77 O10 P1
16	752	-	C47 H90 O6, C48 H94 O5	C40 H79 O10 P1
17	763	-	-	C42 H84 N1 O8 P1, C33 H63 O17 P1
18	780	-	C49 H94 O6, C50 H98 O5	C42 H83 O10 P1
19	794	-	C50 H96 O6, C51 H100 O5	C43 H85 O10 P1
20	805	-	-	C45 H90 N1 O8 P1
21	806	- -	C51 H96 O6, C52 H100 O5	C44 H85 O10 P1
22	850	-	C54 H104 O6, C55 H108 O5	C44 H81 O13 P1
23	852	-	-	C44 H83 O13 P1
24	854	-	-	C44 H85 O13 P1
25	873	-	-	-
26	911	-	-	C38 H71 O22 P1
27	930	-	C60 H112 O6	-
28	934	-	C60 H116 O6	-
29	1008	-	-	C50 H87 O18 P1
30	1044	-	C68 H130 O6	C52 H99 O18 P1
31	1058	-	C69 H132 O6	-

# C50 H82 O1: 1 prenol lipid; C40 H81 O9 P1: 1 polyketide; C50 H83 O4 P1: 1 prenol lipid; C47 H77 N5 O10: 1 polyketide.