Journal of Pathogens

Research Article

Investigations into Isoniazid Treated Mycobacterium tuberculosis by Electrospray Mass Spectrometry Reveals New Insights into Its Lipid Composition

Table 2

Names of the lipids corresponding to the observed m/z values, obtained from Mtb LipidDB (MS-LAMP).

(a)


	Control (Untreated)
S. No.	*Observed m/z* [M+H]**⁺	Fatty Acyls (FAs)	Glycerolipids (GLs)	Glycerophospholipids (GPLs)

1	317	-	MG	-

2	331	-	MG	-

3	409	Mycolipenic acid (C27)	-	-

4	485	-	MG	Lyso-GP

5	497	-	-	Lyso-GP

6	513	-	DG	Lyso-GP

7	539	-	DG	-

8	611	-	-	Lyso-PI

9	626	-	DG	-

10	650	-	DG	-

11	694	Hydroxyphthioceranic acid (C46)	DG	-

12	706	Phthioceranic acid (C48)	DG	-

13	872	-	TG	-

14	1054	-	TG	-

15	1240	DIM-B, Methoxy-MA	TG	Ac1PIM1

MG: monoacylglycerols; DG: diacylglycerols; TG: triacylglycerols; PG: diacylglycerophosphoglycerols; PI: diacylglycerophosphoinositols; PE: diacylglycerolphosphoethanolamines; PIM: phosphatidylinositol mannosides; PIM1: phosphatidylinositol monomannosides; PIM2: phosphatidylinositol dimannoside; DIM-B: phthiodiolone dimycocerosates; MA: mycolic acids; Lyso-GP: monoacylglycerophosphoglycerols; Lyso-PI: monoacylglycerophosphoinositols; Ac1PIM1: monoacylated diacylglycerophosphoinositol monomannosides.

(b)


	INH – Treated
S.No.	*Observed m/z* [M+H]**⁺	Fatty Acyls (FAs)	Glycerolipids (GLs)	Glycerophospholipids (GPLs)

1	343	-	MG	-

2	369	Mycosanoic acid (C24), Mycocerosic acid (C24)	-	-

3	411	Mycocerosic acid (C27)	-	-

4	413	Mycolipanolic acid (C26)	MG	-

5	425	Mycocerosic acid (C28)	-	-

6	467	Mycocerosic acid (C31), Phthioceranic acid (C31)	-	-

7	483	Hydroxy phthioceranic acid (C31)	-	Lyso-GP

8	496	Phthioceranic acid (C33), Mycocerosic acid (C33)	-	Lyso-PE

9	511	-	-	Lyso-GP

10	512	Hydroxy phthioceranic acid (C33)	-	-

11	551	-	DG	-

12	624	-	DG	-

13	700	-	-	-

14	734	-	DG	PG

15	738	-	DG, TG	PG

16	752	-	DG, TG	PG

17	763	-	-	PE, Lyso-PIM1

18	780	-	DG, TG	PG

19	794	-	DG, TG	PG

20	805	-	-	PE

21	806	-	DG, TG	PG

22	850	-	DG, TG	PI

23	852	-	-	PI

24	854	-	-	PI

25	873	-	-	-

26	911	-	-	Lyso-PIM2

27	930	-	TG	-

28	934	-	TG	-

29	1008	-	-	PIM1

30	1044	-	TG	PIM1

31	1058	-	TG	-

decaprenol (1 prenol lipid); phosphomycoketide (polyketide); decaprenyl phosphate (1 prenol lipid); mycobactin w/o Fe (1 polyketide).
MG: monoacylglycerols; DG: diacylglycerols; TG: triacylglycerols; PG: diacylglycerophosphoglycerols; PI: diacylglycerophosphoinositols; PE: diacylglycerolphosphoethanolamines; PIM: phosphatidylinositol mannosides; PIM1: phosphatidylinositol monomannosides; PIM2: phosphatidylinositol dimannoside; DIM-B: phthiodiolone dimycocerosates; MA: mycolic acids; Lyso-GP: monoacylglycerophosphoglycerols; Lyso-PI: monoacylglycerophosphoinositols; Lyso-PIM1: monoacylglycerophosphoinositol monomannosides; Lyso-PIM2: monoacylglycerophosphoinositol dimannosides.