Stress-Adaptive Responses Associated with High-Level Carbapenem Resistance in KPC-Producing Klebsiella pneumoniae

Figure 4

Transcriptional changes in glycolytic pathway: upper glycolysis (section 1, lines 1–11), lower glycolysis: pyruvate and acetate (section 3, lines 38–44), the tricarboxylic acid (TCA) pathway (section 2, lines 12–37), and the glyoxylate shunt (section 4, lines 45–48) after lethal imipenem exposure. At 8 h of imipenem exposure, both BR7 and BR21 strains exhibit a similar metabolic transcriptional profile. Illustrations to guide the reader through these pathways are provided in cited research [32, 33]. Color scale shows gene expression of log₂ fold change (FC) from low (blue) to high (red) expression in imipenem-exposed compared to unexposed bacterial populations (three biological replicates in each sample group). A false discovery rate value correction (FDR) ≤ 0.05 was applied.