Nonphotodynamic Roles of Methylene Blue: Display of Distinct Antimycobacterial and Anticandidal Mode of Actions

Figure 6

Effect of MB on biofilm formation. (a). Upper panel depicts the effect of MB on biofilm formation of C. albicans on polystyrene surface shown as bar graph and quantified by using MTT assay as described in material and methods. OD₄₅₀ nm is depicted on -axis and depicts value < 0.05. Lower panel depicts the effect of MB on biofilm biomass formed on silicone sheets. Biofilm dry weight is depicted on -axis. Inset represents inhibition in biofilm with fluorescence microscopy images of CFW stained cells in presence of MB. (b). Upper panel depicts the effect of MB on biofilm formation of M. smegmatis on polystyrene surface shown as bar graph and quantified by using MTT assay as described in material and methods. OD₄₅₀ nm is depicted on -axis and depicts value < 0.05. Lower panel depicts the effect of MB on biofilm biomass formed on silicone sheets. Biofilm dry weight is depicted on -axis. Inset represents inhibition in biofilm with fluorescence microscopy images of CFW stained cells in presence of MB.

(a)

(b)