Prevalence of Panton-Valentine Leukocidin Gene among Community Acquired Staphylococcus aureus: A Real-Time PCR Study

Figure 3

(a) Agarose gel electrophoresis pattern for identification of Staphylococcus aureus specific 16S rRNA gene. M, molecular weight marker. Lane 1-13, different clinical strains of Staphylococcus aureus. Lane 14, negative control, Escherichia coli (SM10 λpir). (b) nuc gene. M, DNA molecular weight marker (100 bp DNA ladder); Lane 1, positive control; Lanes 2, 3, 4, and 5, 270 bp band obtained with DNA from clinical strains. Lane 6, negative control (Staphylococcus epidermidis). (c) Agarose gel electrophoresis patterns showing PCR-amplified products for the mecA gene. M, DNA molecular weight marker (100 bp DNA ladder). Lane 1, positive control; Lanes 2, 3, 4, 5, and 6, 147 bp band obtained with DNA from MRSA clinical strains. Lane 7, negative control (Staphylococcus epidermidis). (d) Agarose gel electrophoresis patterns showing PCR-amplified products for the pvl gene. Lanes 1 and 2, 433 bp band obtained with DNA from MRSA clinical strains.

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