Invasion of Epithelial Cells Is Correlated with Secretion of Biosurfactant via the Type 3 Secretion System (T3SS) of Shigella flexneri

Figure 5

BLM purified from Shigella sp. TOP: profile obtained after precipitation with ethanol (CH₃CH₂OH), hydrochloric acid (HCl), and ammonium sulfate ((NH₄)₂SO₄). EI24: emulsification index for all strains. S. flexneri M90T, S. sonnei, S. boydii, and Shigella sp.: Ssp1, 2, and 3. Bottom panel: residues obtained after evaporation of chloroform (CCl₄) (left); emulsification index (EI24) for the extractable biosurfactant-like molecule (right). Bios1 and Bios2: biosurfactant-like molecule residues.

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