Investigating the Role of Mucin as Frontline Defense of Mucosal Surfaces against Mycobacterium avium Subsp. hominissuis

Table 5

Effect of Tween-20 and trypsin treatment on the binding to the MUC2 mucin.

Bacteria	% binding to MUC2 mucin
	Control (7H9)	Tween-20	Trypsin
M. avium 104	0	47 ± 6	38.1 ± 4
M. avium 3388	0	54 ± 6	48.4 ± 5

compared to M. avium incubated in the 7H9 broth. M. avium strains were treated with Tween-20 or trypsin for 3 h at 37°C, washed, and incubated with 5% MUC2 mucin. The numbers represent the percent of binding to the mucin.