Investigating the Role of Mucin as Frontline Defense of Mucosal Surfaces against Mycobacterium avium Subsp. hominissuis

Table 7

Effect of polymyxin B on the invasion of HEp-2 cells in presence of the MUC2 mucin.

Bacteria	Polymyxin B	Invasion HEp-2	Invasion HEp-2 + polymyxin B
M. avium 104	4.5 ± 0.5 × 105	8.3 ± 0.4 × 104	8.0 ± 0.5 × 104
MAH 4B2	2.5 ± 0.6 × 103	3.2 ± 0.5 × 103	6.4 ± 0.4 × 102

Inoculum was 4.5 × 10⁵ for M. avium and 3 × 10⁵ for MAH 4B2. Bacteria were incubated with polymyxin for 4 hours, and the diluted and plated bacteria were placed on the monolayers with or without polymyxin for 2 h; then extracellular bacteria were removed, and HEp-2 cells were lysed. The lysate was plated for quantification of colony forming units. for the comparison between M. avium 104 and 4B2. for the comparison between MAH 4B2 invasion of HEp-2 cells with and without polymyxin B.