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Journal of Parasitology Research
Volume 2009 (2009), Article ID 165812, 7 pages
Research Article

Vaccine Efficacy of Bm86 Ortholog of H. a. anatolicum, rHaa86 Expressed in Prokaryotic Expression System

1Centre for Biosystem Research, University of Maryland Biotechnology Institute, Rockville, MD 20742-4450, USA
2Entomology Laboratory, Parasitology Division, Indian Veterinary Research Institute, Izatnagar, 243122 Uttar Pradesh, India
3Division of Bacteriology and Mycology, Indian Veterinary Research Institute, Izatnagar, 243122 Uttar Pradesh, India

Received 11 September 2009; Accepted 30 November 2009

Academic Editor: Barbara Papadopoulou

Copyright © 2009 P. Azhahianambi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The use of tick vaccine in controlling ticks and tick borne diseases has been proved effective in integrated tick management format. For the control of H. a. anatolicum, Bm86 ortholog of H. a. anatolicum was cloned and expressed as fusion protein in E. coli as E. coli-pETHaa86. The molecular weight of the rHaa86 was 97 kDa with a 19 kDa fusion tag of thioredoxin protein. The expressed protein was characterized immunologically and vaccine efficacy was evaluated. After 120 hours of challenge, only 26% tick could successfully fed on immunized animals. Besides significant reduction in feeding percentages, a significant reduction of 49.6 mg ; in the weight of fed females in comparison to the females fed on control animals was recorded. Following oviposition, a significant reduction of 68.1 mg; in the egg masses of ticks fed on immunized animals in comparison to the ticks fed on control animals was noted. The reduction of number of females, mean weight of eggs, adult females and efficacy of immunogen were 73.8%, 31.3%, 15.8%, and 82.3%, respectively. The results indicated the possibility of development of rHaa86 based vaccine as a component of integrated control of tick species.