Genotoxicity Revaluation of Three Commercial Nitroheterocyclic Drugs: Nifurtimox, Benznidazole, and Metronidazole

<table>DNA damage detected in human leukocytes treated (37°C, 1 hour) with NFX, BNZ, and MTZ. (a): DNA damage is expressed as total migration length (TL, <svg height="9.6750002" id="M56" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,9.675)">
<g transform="translate(72,-64.26)">
<text transform="matrix(1,0,0,-1,-71.95,66.6)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝜇</tspan>
</text>
</g>
</g>
</svg>m) detected by the alkaline comet assay. (b) Specific oxidatively generated damage to DNA detected by the modified comet assay (ENDO III). DNA oxidative damage is expressed as DNA migration <svg height="13.55" id="M57" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 14.85 13.55" width="14.85" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,13.55)">
<g transform="translate(72,-61.16)">
<text transform="matrix(1,0,0,-1,-71.95,63.5)">
<tspan style="font-size: 12.50px; " x="0" y="0">(</tspan>
<tspan style="font-size: 12.50px; " x="4.1634989" y="0">𝜇</tspan>
</text>
</g>
</g>
</svg>m) increase with respect to the assay without enzymes. Mean ± SD of three independent experiments. Filled symbols, <svg height="10.325" id="M58" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
</text>
</g>
</g>
</svg> &lt; .05 (Dunnett’s C) with respect to dose 0.</table>

Journal of Parasitology Research

fig2

Figure 2

Figure 2: Genotoxicity Revaluation of Three Commercial Nitroheterocyclic Drugs: Nifurtimox, Benznidazole, and Metronidazole 