Genotoxicity Revaluation of Three Commercial Nitroheterocyclic Drugs: Nifurtimox, Benznidazole, and Metronidazole

Table 3

Mean frequency of Micronuclei (MN) in binucleated cells and Nuclear Division Index (NDI) in human lymphocytes treated with NFX (24 hours) or BNZ (72 hours) Positive controls:- ethyl methane sulfonate (1 𝜇M):15.0 ± 2.8; bleomycin (4 𝜇M): 26.0 ± 4.0; demecolcine (0.9 𝜇M): 65.0 ± 3.3.

NFX (24 hours)				BNZ (72 hours)
Dose	NDI	NDI	MN	Dose	NDI	NDI	MN
( 𝝁 M)		(%)	(×10^-3 BN)	( 𝝁 M)		(%)	(×10^-3 BN)
0	1.97 ± 0.10	100	2.0 ± 0.5	0	1.93 ± 0.10	100	3.5 ± 0.5
1.7	1.95 ± 0.12	99 ± 6	2.5 ± 0.5	1.9	1.97 ± 0.10	102 ± 5	3.0 ± 0.5
3.5	1.97 ± 0.07	100 ± 4	2.0 ± 1.0	3.8	1.93 ± 0.11	100 ± 6	3.5 ± 1.0
7.0	1.89 ± 0.05	96 ± 3	3.0 ± 1.0	7.7	2.00 ± 0.07	104 ± 4	4.0 ± 1.0
13.9	1.85 ± 0.06	94 ± 3	2.5 ± 0.5	15.4	1.98 ± 0.06	102 ± 3	4.0 ± 1.5
27.9	1.75 ± 0.10	89 ± 5	3.0 ± 0.5	30.7	1.99 ± 0.05	103 ± 3	3.5 ± 1.0
55.7	1.69 ± 0.04^𝐚	86 ± 2^𝐚	3.0 ± 1.0	61.5	2.06 ± 0.07	107 ± 4	3.0 ± 0.5
87.0	1.61 ± 0.10^𝐚	82 ± 5^𝐚	4.0 ± 0.5	96.1	2.05 ± 0.03	106 ± 2	9.0 ± 0.5
174.0	1.43 ± 0.03^𝐚	73 ± 2^𝐚	9.0 ± 1.0	192.1	2.00 ± 0.07	104 ± 4	13.0 ± 1.0^𝐛
348.1	1.41 ± 0.02^𝐚	72 ± 1^𝐚	12.0 ± 1.0^𝐛	384.2	1.93 ± 0.10	100 ± 6	16.5 ± 0.5^𝐛

^𝐚𝑃<.05 Student’s 𝑡-test; ^𝐛𝑃<.052χ-test