In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid of Echinococcus granulosus

Figure 1

Morphological aspects of cultured PSCs in presence of exogenous nitric oxide metabolites. (a) Cultured invaginated PSCs at 𝑡=0 (initial PSCs viability = 98%); (b) cultured PSCs after 48 hours of incubation without any treatment, (c) after 48 hours of incubation with 320 𝜇M of NO₃₋, (d) after 24 hours of incubation with 320 𝜇M of NO₂₋, (e) after 3 hours of incubation with 80 𝜇M of ONOO⁻, and (f) 1 hour of incubation with 160 𝜇M of ONOO⁻. (H): Hooks; (CH): Crown of hooks; (SU): Suckers; (I.PSC): invaginated protoscolices; (E.PSC): evaginated protoscolices; (T. PSC): Turgescent protoscolices; (R.PSC): retracted protoscolices; (): Alteration of tegument; (Res): Residues of dead protoscolices.

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