Table of Contents Author Guidelines Submit a Manuscript
Journal of Parasitology Research
Volume 2009, Article ID 680790, 6 pages
Research Article

Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral Leishmaniasis

1Departamento de Protozoologia, FIOCRUZ, Rio de Janeiro, CEP 21040-360, Brazil
2Centro de Ciências de Saúde, UFRJ, Rio de Janeiro, CEP 21941-901, Brazil
3Laboratório de Bioquímica Molecular de Doenças Endêmicas, FIOCRUZ, Rio de Janeiro, CEP 21040-360, Brazil
4Laboratório de Bioquímica e Fisiologia de Insetos, FIOCRUZ, Rio de Janeiro, CEP 21040-360, Brazil

Received 14 July 2009; Accepted 16 October 2009

Academic Editor: David S. Lindsay

Copyright © 2009 Alba Valéria Machado da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB). After twelve months, the results obtained from IFA demonstrated that 50% were seropositive and two serological profiles were observed: the first one ranging from 1/40 to 1/80 and the second 1/160. By WB, it was observed that the same percentage and sera from positive dogs presented the recognition of the peptides of 29 and 32 kDa up to 8 months before IFA serum conversion. Among the positive dogs, all the sera from symptomatic ones with tissue parasitism recognized the peptide of 68.5 kDa. Our results suggest the need of modifications in the control measures regarding the elimination of the dogs. They also corroborate the high sensitivity and specificity of western blot in the diagnosis of canine leishmaniasis, suggesting the possibility of its association with IFA.