Figure 2: Antigenic recognition by western blot from sera of symptomatic and asymptomatic dogs with L. (i.) chagasi. Antigen recognition of Leishmania (i.) chagasi by serum from naturally infected dogs. Lane (−): serum from serumnegative (IFA) dog. Lane (+): serum from serumpositive (IFA 1/1280) dog. Lanes a and b: IFA seropositive symptomatic dogs. Lanes c, d, and e: IFA seropositive asymptomatic dogs.