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Journal of Parasitology Research
Volume 2010, Article ID 517534, 7 pages
Research Article

Level of CYP4G19 Expression Is Associated with Pyrethroid Resistance in Blattella germanica

1Department of Pathogenic Organism, Fourth Military Medical University, Xian 710032, China
2Shenzhen the Fifth the People Hospital, Shenzhen, China
3Shenzhen Centre for Diseases Control and Prevention, Shenzhen 518020, China

Received 30 September 2009; Revised 16 December 2009; Accepted 26 January 2010

Academic Editor: Xin-zhuan Su

Copyright © 2010 Guang-zhou Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


German cockroaches have become a large problem in the Shenzhen area because of their pesticide resistance, especially to pyrethroid. A pyrethroid called “Jia Chong Qing” to prevent pests for a long time were found to be resistant to “Jia Chong Qing” with resistance index of 3.88 measured using RT-PCR and immunohistochemistry analysis showed that both CYP4G19 mRNA and CYP4G19 protein expression levels in the wild strain were substantially higher than that of a sensitive strain. dsRNA segments derived from the target gene CYP4G19 were prepared using in vitro transcription and were microinjected into abdomens of the wild strain. Two to eight days after injection, the result showed that CYP4G19 mRNA expressions were significantly reduced in the groups injected with dsRNAs.