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Journal of Parasitology Research
Volume 2011, Article ID 129542, 2 pages
Research Article

Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children

1Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria
2Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
3Department of Zoology, Lagos State University, Ojo, Lagos, Nigeria

Received 21 July 2011; Accepted 14 September 2011

Academic Editor: Takeshi Agatsuma

Copyright © 2011 Adekunle Bamidele Ayinmode et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A study was conducted to detect and identify enteric microsporidian species in 43 children from Oyo state, Nigeria. Using nested polymerase chain reaction, 9.3% of the children were identified as positive for Enterocytozoon bieneusi. DNA sequencing of the PCR products showed the presence of three known genotypes (two isolates of genotype D and one of genotype K) and one new genotype. This study suggests that either human or animal (or both) could be the infection source for the children, since identified genotypes D and K have been previously detected in both immunocompromised and immunocompetent patients and domestic animals. The identification of high diversity also suggests intensive transmission of microsporidiosis in the studied area.