Research Article

An ELISA to Detect Serum Antibodies to the Salivary Gland Toxin of Ixodes holocyclus Neumann in Dogs and Rodents

Figure 3

The reaction of rat sera to Ixodes antigen in ELISA after two exposures to I. holocyclus ticks. Preexposure (negative) and postexposure (positive) rat serum was titrated in doubling dilutions and tested against Ixodes and Rhipicephalus antigen in ELISA using the optimal conditions and protocol described earlier.
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