Trypanosoma congolense Infections: Induced Nitric Oxide Inhibits Parasite Growth In Vivo
Figure 5
Higher rate of multiplication of T. congolense in infected iNOS−/− mice than in infected wild-type mice. Groups of 6 WT C57BL/6 and iNOS−/− mice were infected with T. congolense TC13. The percentage of trypanosomes being in theG1 and S/G2/M phases was determined by DNA FACS analysis (see Materials and Methods). (a) A typical FACS DNA pattern of T. congolense freshly isolated from infected immunosuppressed mice. (b) A typical FACS DNA pattern of T. congolense in infected iNOS−/− mice at day 6 postinfection. (c) A typical FACS DNA pattern of T. congolense in infected WT C57BL/6 mice. (d) Kinetics of S/G2/M phases of trypanosomes in WT and iNOS−/− mice following TC13 infection. Note: on days 5 and 6, the trypanosomes in iNOS−/− mice had a significantly higher ratio of organisms in the S/G2/M phases than trypanosomes in WT mice.
