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Journal of Parasitology Research
Volume 2012, Article ID 278028, 6 pages
http://dx.doi.org/10.1155/2012/278028
Clinical Study

Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo

1Département de Médecine Tropicale, Maladies Infectieuses et Parasitaires, Cliniques Universitaires de Kinshasa, Université de Kinshasa, 747 Kinshasa XI, Democratic Republic of Congo
2Service de Parasitologie-Mycologie, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris et Faculté de Médecine Lariboisière-Saint-Louis, Université Paris VII, 75010 Paris, France
3Faculty of Health Sciences, Walter Sisulu University, Private Bag XI, Mthatha, Eastern Cape 5117, South Africa
4Department of Internal Medicine, University of Kinshasa, 783 Kinshasa XI, Democratic Republic of Congo
5National Center for Malaria Research, AP-HP, CHU Pitié Salpetrière, 75013 Paris, France
6AP-HP, Groupe hospitalier Pitié-Salpêtrière, Service de Parasitologie-Mycologie, Université Pierre et Marie Curie, 75013 Paris, France

Received 19 March 2012; Revised 10 April 2012; Accepted 24 April 2012

Academic Editor: Francisca Mutapi

Copyright © 2012 Roger Wumba et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. Weber, R. T. Bryan, D. A. Schwartz, and R. L. Owen, “Human microsporidial infections,” Clinical Microbiology Reviews, vol. 7, no. 4, pp. 426–461, 1994. View at Google Scholar · View at Scopus
  2. D. M. Asmuth, P. C. DeGirolami, M. Federman et al., “Clinical features of microsporidiosis in patients with AIDS,” Clinical Infectious Diseases, vol. 18, no. 5, pp. 819–825, 1994. View at Google Scholar · View at Scopus
  3. D. P. Kotler and J. M. Orenstein, “Clinical syndromes associated with microsporidiosis,” in The Microsporidia and Microsporidiosis, M. Wittner and L. M. Weiss, Eds., pp. 258–292, ASM Press, Washington, DC, USA, 1999. View at Google Scholar
  4. I. Maiga, O. Doumbo, M. Dembele et al., “Human intestinal microsporidiosis in Bamako (Mali): the presence of Enterocytozoon bieneusi in HIV seropositive patients,” Cahiers Santé, vol. 7, no. 4, pp. 257–262, 1997. View at Google Scholar · View at Scopus
  5. O. Liguory, F. David, C. Sarfati et al., “Diagnosis of infections caused by Enterocytozoon bieneusi and Encephalitozoon intestinalis using polymerase chain reaction in stool specimens,” AIDS, vol. 11, no. 6, pp. 723–726, 1997. View at Google Scholar · View at Scopus
  6. E. S. Didier, J. M. Orenstein, A. Aldras, D. Bertucci, L. B. Rogers, and F. A. Janney, “Comparison of three staining methods for detecting microsporidia in fluids,” Journal of Clinical Microbiology, vol. 33, no. 12, pp. 3138–3145, 1995. View at Google Scholar · View at Scopus
  7. D. P. Kotler and J. M. Orenstein, “Prevalence of intestinal microsporidiosis in HIV-infected individuals referred for gastroenterological evaluation,” American Journal of Gastroenterology, vol. 89, no. 11, pp. 1998–2002, 1994. View at Google Scholar · View at Scopus
  8. R. Wumba, A. Enache-Angoulvant, M. Develoux et al., “Prévalence des infections opportunistes digestives parasitaires à kinshasa (république démocratique du congo), résultats d’une enquête préliminaire chez 50 patients au stade sida,” Revue Médecine Tropicale, vol. 67, no. 2, pp. 145–148, 2007. View at Google Scholar · View at Scopus
  9. R. Wumba, B. Longo-Mbenza, M. Mandina et al., “Intestinal parasites infections in hospitalized aids patients in kinshasa, democratic Republic Of Congo,” Parasite, vol. 17, no. 4, pp. 321–328, 2010. View at Google Scholar · View at Scopus
  10. J. Menotti, B. Cassinat, R. Porcher, C. Sarfati, F. Derouin, and J. M. Molina, “Development of a real-time polymerase-chain-reaction assay for quantitative detection of Enterocytozoon bieneusi DNA in stool specimens from immunocompromised patients with intestinal microsporidiosis,” Journal of Infectious Diseases, vol. 187, no. 9, pp. 1469–1474, 2003. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Menotti, B. Cassinat, C. Sarfati, O. Liguory, F. Derouin, and J. M. Molina, “Development of a real-time PCR assay for quantitative detection of Encephalitozoon intestinalis DNA,” Journal of Clinical Microbiology, vol. 41, no. 4, pp. 1410–1413, 2003. View at Publisher · View at Google Scholar · View at Scopus
  12. O. Liguory, F. David, C. Sarfati, F. Derouin, and J. M. Molina, “Determination of types of Enterocytozoon bieneusi strains isolated from patients with intestinal microsporidiosis,” Journal of Clinical Microbiology, vol. 36, no. 7, pp. 1882–1885, 1998. View at Google Scholar · View at Scopus
  13. O. Liguory, C. Sarfati, F. Derouin, and J. M. Molina, “Evidence of different Enterocytozoon bieneusi genotypes in patients with and without human immunodeficiency virus infection,” Journal of Clinical Microbiology, vol. 39, no. 7, pp. 2672–2674, 2001. View at Publisher · View at Google Scholar · View at Scopus
  14. R. Weber, R. T. Bryan, R. L. Owen, C. M. Wilcox, L. Gorelkin, and G. S. Visvesvara, “Improved light-microscopical detection of microsporidia spores in stool and duodenal aspirates,” The New England Journal of Medicine, vol. 326, no. 3, pp. 161–166, 1991. View at Google Scholar · View at Scopus
  15. O. A. Cisse, A. Ouattara, M. Thellier et al., “Evaluation of an immunofluorescent-antibody test using monoclonal antibodies directed against Enterocytozoon bieneusi and Encephalitozoon intestinalis for diagnosis of intestinal microsporidiosis in bamako (Mali),” Journal of Clinical Microbiology, vol. 40, no. 5, pp. 1715–1718, 2002. View at Publisher · View at Google Scholar · View at Scopus
  16. I. Accoceberry, M. Thellier, I. Desportes-Livage et al., “Production of monoclonal antibodies directed against the microsporidium Enterocytozoon bieneusi,” Journal of Clinical Microbiology, vol. 37, no. 12, pp. 4107–4112, 1999. View at Google Scholar · View at Scopus
  17. C. Sarfati, A. Bourgeois, J. Menotti et al., “Prevalence of intestinal parasites including microsporidia in human immunodeficiency virus-infected adults in Cameroon: a cross-sectional study,” The American Journal of Tropical Medicine and Hygiene, vol. 74, no. 1, pp. 162–164, 2006. View at Google Scholar · View at Scopus
  18. P. Kadende, T. Nkurunziza, J. J. Floch et al., “Infectious diarrhoea conconmitant with African AIDS. Review of 100 cases observed in Bujumbura (Burundi),” Medecine Tropicale, vol. 49, no. 2, pp. 129–213, 1989. View at Google Scholar · View at Scopus
  19. F. Drobniewski, P. Kelly, A. Carew et al., “Human microsporidiosis in African AIDS patients with chronic diarrhea,” Journal of Infectious Diseases, vol. 171, no. 2, pp. 515–516, 1995. View at Google Scholar · View at Scopus
  20. P. Kelly, S. E. Davies, B. Mandanda et al., “Enteropathy in Zambians with HIV related diarrhoea: regression modelling of potential determinants of mucosal damage,” Gut, vol. 41, no. 6, pp. 811–816, 1997. View at Google Scholar · View at Scopus
  21. T. Gumbo, S. Sarbah, I. T. Gangaidzo et al., “Intestinal parasites in patients with diarrhea and human immunodeficiency virus infection in Zimbabwe,” AIDS, vol. 13, no. 7, pp. 819–821, 1999. View at Publisher · View at Google Scholar · View at Scopus
  22. T. van Gool, E. Luderhoff, K. J. Nathoo, C. F. Kiire, J. Dankert, and P. R. Mason, “High prevalence of Enterocytozoon bieneusi infections among HIV-positive individuals with persistent diarrhoea in Harare, Zimbabwe,” Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 89, no. 5, pp. 478–480, 1995. View at Publisher · View at Google Scholar · View at Scopus
  23. Y. Dieng, T. H. Dieng, G. Diouf, A. M. Coll-Seck, and S. Diallo, “Screening for microsporidian spores in AIDS patients at the fann University Hospital, Dakar (Senegal): preliminary results,” Medecine et Maladies Infectieuses, vol. 28, no. 3, pp. 265–267, 1998. View at Google Scholar · View at Scopus
  24. M. Lebbad, H. Norrgren, A. Nauclér, F. Dias, S. Andersson, and E. Linder, “Intestinal parasites in HIV-2 associated AIDS cases with chronic diarrhoea in Guinea-Bissau,” Acta Tropica, vol. 80, no. 1, pp. 45–49, 2001. View at Publisher · View at Google Scholar · View at Scopus
  25. A. Samé-Ekobo, J. Lohoué, and A. Mbassi, “Clinical and biological study of parasitic and fungal diarrhea in HIV patients in the urban and suburban areas of Yaounde,” Cahiers Sante, vol. 7, no. 6, pp. 349–354, 1997. View at Google Scholar · View at Scopus
  26. V. Le Moing, F. Bissuel, D. Costagliola et al., “Decreased prevalence of intestinal cryptosporidiosis in HIV-infected patients concomitant to the widespread use of protease inhibitors,” AIDS, vol. 12, no. 11, pp. 1395–1397, 1998. View at Publisher · View at Google Scholar · View at Scopus
  27. A. Carr, D. Marriott, A. Field, E. Vasak, and D. A. Cooper, “Treatment of HIV-1-associated microsporidiosis and cryptosporidiosis with combination antiretroviral therapy,” The Lancet, vol. 351, no. 9098, pp. 256–261, 1998. View at Publisher · View at Google Scholar · View at Scopus
  28. C. Mwachari, B. I. F. Batchelor, J. Paul, P. G. Waiyaki, and C. F. Gilks, “Chronic diarrhoea among HIV-infected adult patients in Nairobi, Kenya,” Journal of Infection, vol. 37, no. 1, pp. 48–53, 1998. View at Publisher · View at Google Scholar · View at Scopus
  29. J. K. Tumwine, A. Kekitiinwa, N. Nabukeera, D. E. Akiyoshi, M. A. Buckholt, and S. Tzipori, “Enterocytozoon bieneusi among children with diarrhea attending Mulago hospital in Uganda,” The American Journal of Tropical Medicine and Hygiene, vol. 67, no. 3, pp. 299–303, 2002. View at Google Scholar · View at Scopus
  30. M. Santín and R. Fayer, “Enterocytozoon bieneusi genotype nomenclature based on the internal transcribed spacer sequence: a consensus,” Journal of Eukaryotic Microbiology, vol. 56, no. 1, pp. 34–38, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. J. Breton, E. Bart-Delabesse, S. Biligui et al., “New highly divergent rRNA sequence among biodiverse genotypes of Enterocytozoon bieneusi strains isolated from humans in Gabon and Cameroon,” Journal of Clinical Microbiology, vol. 45, no. 8, pp. 2580–2589, 2007. View at Publisher · View at Google Scholar · View at Scopus
  32. F. O. Akinbo, C. E. Okaka, R. Omoregie, T. Dearen, E. T. Leon, and L. Xiao, “Molecular characterization of Cryptosporidium spp. in HIV-infected persons in Benin City, Edo State, Nigeria,” The The American Journal of Tropical Medicine and Hygiene, vol. 86, no. 3, pp. 441–445, 2012. View at Publisher · View at Google Scholar
  33. M. L. Lobo, L. Xiao, F. Antunes, and O. Matos, “Microsporidia as emerging pathogens and the implication for public health: a 10-year study on HIV-positive and -negative patients,” International Journal for Parasitology, vol. 42, pp. 197–205, 2012. View at Google Scholar
  34. H. Rinder, S. Katzwinkel-Wladarsch, and T. Löscher, “Evidence for the existence of genetically distinct strains of Enterocytozoon bieneusi,” Parasitology Research, vol. 83, no. 7, pp. 670–672, 1997. View at Publisher · View at Google Scholar · View at Scopus
  35. R. A. Hartskeerl, A. R. J. Schuitema, T. Van Gool, and W. J. Terpstra, “Genetic evidence for the occurrence of extra-intestinal Enterocytozoon bieneusi infections,” Nucleic Acids Research, vol. 21, no. 17, article 4150, 1993. View at Google Scholar · View at Scopus
  36. J. M. Molina, J. Goguel, C. Sarfati et al., “Potential efficacy of fumagillin in intestinal microsporidiosis due to Enterocytozoon bieneusi in patients with HIV infection: results of a drug screening study,” AIDS, vol. 11, no. 13, pp. 1603–1610, 1997. View at Publisher · View at Google Scholar · View at Scopus
  37. S. Pol, C. A. Romana, S. Richard et al., “Microsporidia infection in patients with the human immunodeficiency virus and unexplained cholangitis,” The New England Journal of Medicine, vol. 328, no. 2, pp. 95–99, 1992. View at Publisher · View at Google Scholar · View at Scopus
  38. Y. Hutin, M. N. Sombardier, C. Sarfati, J. M. Decazes, J. Modai, and J. M. Molina, “Risk factors for intestinal microsporidiosis in patients infected with human immunodeficiency virus (HIV),” in Proceedings of the 37th Interscience Conference on Antimicrobial Agents and Chemotherapy, pp. 1–150, American Society for Microbiology, September 1997, Abstracts.
  39. B. Swaminathan and T. J. Barrett, “Amplification methods for epidemiologic investigations of infectious diseases,” Journal of Microbiological Methods, vol. 23, no. 1, pp. 129–139, 1995. View at Publisher · View at Google Scholar · View at Scopus