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Journal of Parasitology Research
Volume 2012 (2012), Article ID 394981, 10 pages
http://dx.doi.org/10.1155/2012/394981
Research Article

Cytokine and Chemokine Profile in Individuals with Different Degrees of Periportal Fibrosis due to Schistosoma mansoni Infection

1Serviço de Imunologia, Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, Brazil
2Departamento de Ciências da Vida, Universidade do Estado da Bahia, 2555 Rua Silveira Martins, Cabula, 41.150-000 Salvador, BA, Brazil
3Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT-CNPQ/MCT), Rua João das Botas s/n, Canela, 40.110-160 Salvador, BA, Brazil
4Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, UFBA, Avenida Adhemar de Barros s/n, Ondina, 40.170-970 Salvador, BA, Brazil
5Escola Bahiana de Medicina e Saúde Pública, Rua Frei Henrique No. 08, Nazaré, 40.050-420 Salvador, BA, Brazil

Received 13 July 2012; Accepted 7 November 2012

Academic Editor: Sergio Costa Oliveira

Copyright © 2012 Robson Da Paixão De Souza et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Periportal fibrosis in schistosomiasis has been associated to the host immune response to parasite antigens. We evaluated the immune response in S. mansoni infected individuals with different degrees of periportal fibrosis. Cytokine and chemokines were measured in serum and in supernatants of PBMC cultures stimulated with the soluble adult worm (SWAP) or egg (SEA) antigens, using a sandwich ELISA. The levels of IL-5 in response to SEA were higher in individuals with moderate to severe fibrosis (310.9 pg/mL) compared to individuals without fibrosis (36.8 pg/mL; ). There was also a higher production of TNF-α in cultures stimulated with SWAP in patients with insipient fibrosis (1446 pg/mL) compared to those without fibrosis (756.1 pg/mL; ). The serum levels of IL-13 and MIP-1α were higher in subjects without fibrosis than in those with moderate to severe fibrosis. However a positive association between serum levels of IL-13, TNF-α, MIP-1α, and RANTES and S. mansoni parasite burden was found. From these data we conclude that IL-5 and TNF-α may participate in liver pathology in schistosomiasis. The positive association between IL-13, TNF-α, MIP-1α, and RANTES with parasite burden, however, might predict the development of liver pathology.