Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh

Figure 2

RFLP-PCR analyses of 18S rRNA genes of P. argentipes, two Sergentomyia species, and P. papatasi. PCR amplification with Lu.18S 1S and Lu.18S 1R primers was performed to amplify approximately 450 bp fragments of sand fly 18S rRNA gene. The PCR products were digested with HinfI, and the digested samples were separated by electrophoresis in a 3% agarose gel to produce DNA fragments. Lane M; 100-basepair ladder, lane 1; P. argentipes, lane 2; Sergentomyia species A, and lane 3; Sergentomyia species B. Lane 4; P. papatasi.