Changes in T-Cell and Monocyte Phenotypes In Vitro by Schistosoma mansoni Antigens in Cutaneous Leishmaniasis Patients
Phenotype of T cells and co-stimulatory molecules expression on T lymphocytes of CL patients () stimulated in vitro with SLA in the presence or absence of S. mansoni antigens rSm29, rSmTSP-2 or with a fraction of S. mansoni soluble adult worm antigen (SWAP) PIII. Frequency of CD4+ and CD8+ T cells (A), expression of CD28 and CTLA-4 in CD4+ and CD8+ T cells (B and C, resp.) and the frequency of CD4+CD25highFoxp3+ T cells () (D). Cells were stained for surface expression of CD4, CD8, CD25 and CD28, while the expression of CTLA-4 and Foxp3 were evaluated intracellularly using flow cytometry. cultures without stimulation (WS) versus SLA + S. mansoni antigens; SLA versus SLA + S. mansoni antigens (Wilcoxon matched pairs test).