New Insights on the Inflammatory Role of Lutzomyia longipalpis Saliva in Leishmaniasis
Figure 2
Neutrophil influx, apoptosis, and phagocytosis into BALB/c and C57BL/6 peritoneal cavity in response to L. longipalpis saliva. Mice were injected with endotoxin-free saline or L. longipalpis salivary gland sonicate (SGS) (0.5 pair/animal). After stimulation, peritoneal cavities were washed and differential cell counts were performed on Diff-Quik stained cytospin preparations. (a-b) Kinetics of neutrophil recruitment in BALB/c (a) and C57BL/6 (b) mice. (c-d) Representative events of C57BL/6 neutrophil phagocytosis by macrophages on Diff-Quik stained cytospin (magnification 1000x). (e-f) Phagocytosis of C57BL/6 leukocytes by macrophages (e) and neutrophil apoptosis (f) after stimulation with SGS (•) or saline (□). Data shown are from a single experiment representative of three independent experiments. Values represent means ± SEM of five mice per group. and .
