Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene
Figure 2
Flow cytometric analysis of CD4+ and CD8α + cells isolated from splenocytes of chickens. Chickens in each group were, respectively, intramuscularly immunized with 100 μg pcDNA-3-1E-linker-mChIL-15 (group 1), 100 μg pcDNA3.1 (group 2), and sterile TE buffer (pH 7.6) (group 3) at 14 and 21 days of age. All chickens were inoculated orally with 5 × 104 sporulated oocysts of E. acervulina at 28 days of age (day 7 after secondary immunization). The ratio of CD4+ and CD8α + cells in spleen of randomly selected five chickens was assayed at 28 and 34 days of age (day 6 after challenge). Data represent mean ± standard errors ( per group). Highly significant difference () between bars not sharing the same capital letters in each time point. Significant difference () between bars not sharing the same small letters in each time point.
