Research Article

Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene

Table 1

Experimental groups used in immunization and challenge experiments.

GroupNumberImmunizationChallenge at 28 days of age
Primary immunization at 14 days of ageSecondary immunization at 21 days of ageNumber of E. acervulina-sporulated oocysts

125pcDNA-3-1E-mChIL-15 (100 μg)pcDNA3-1E-mChIL-15 (100 μg)5 × 104
225pcDNA3.1 (100 μg)pcDNA3.1 (100 μg)5 × 104
325TE buffer (pH 7.6)TE buffer (pH 7.6)5 × 104