Research Article
Induction of Cellular Immune Response by DNA Vaccine Coexpressing E. acervulina 3-1E Gene and Mature CHIl-15 Gene
Table 1
Experimental groups used in immunization and challenge experiments.
| Group | Number | Immunization | Challenge at 28 days of age | Primary immunization at 14 days of age | Secondary immunization at 21 days of age | Number of E. acervulina-sporulated oocysts |
| 1 | 25 | pcDNA-3-1E-mChIL-15 (100 μg) | pcDNA3-1E-mChIL-15 (100 μg) | 5 × 104 | 2 | 25 | pcDNA3.1 (100 μg) | pcDNA3.1 (100 μg) | 5 × 104 | 3 | 25 | TE buffer (pH 7.6) | TE buffer (pH 7.6) | 5 × 104 |
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