Development of Eimeria nieschulzi (Coccidia, Apicomplexa) Gamonts and Oocysts in Primary Fetal Rat Cells

Table 1

Number and type of experiments performed in this study.

Cells	Coating	Cell growth	Max. parasite development
IPEC-J2 [2]	Uncoated	Monolayer	Sch II/Mz II
ST [2]	Uncoated	Monolayer	Sch II/Mz II
VERO [>2]	Uncoated	Monolayer, later multilayer	Sch II/Mz II
IEC6 [>2]	Uncoated	Monolayer	Sch II/Mz II
Prim.intestine [3]; Prim.intestine [1]; Prim.intestine [1]	Fibronectin; Gelatin; Uncoated	Monolayered fibroblast, degrading cell conglomerates, no growing organoids	Sch II/Mz II
Primary liver [2]	Fibronectin	Monolayer	Sch II/Mz II
Primary kidney [2]	Fibronectin	Monolayer	Sch II/Mz II
Primary fetal mix [3]	Fibronectin	Monolayer and cell conglomerates in two experiments (1., 2.) no growing organoids in one experiments (3.) growing crypt-like organoids	(1.) Sch II/Mz II; (2.) Sch II/Mz II; (3.) Mag/Ooc
Primary fetal mix [2]	Gelatin	Monolayer and cell conglomerates, in one experiment no organoids (1.) in one experiment growing crypt-like organoids (2.)	(1.) Sch II/Mz II; (2.) Mag/Ooc.
Primary fetal mix [1]	Matrigel	Monolayer and growing organoids	Mag/Ooc
Primary fetal mix (3)	Uncoated	Monolayered fibroblast, degrading cell conglomerates, no growing organoids	Sch II/Mz II

[number of experiments]; (experiment number) Sch II: schizont 2nd generation, Mz II merozoite 2nd generation, Mag: macrogamont, Ooc: oocyst.