Improved Method for the Detection and Quantification of Naegleria fowleri in Water and Sediment Using Immunomagnetic Separation and Real-Time PCR

Table 2

IMS recovery efficiency for non-N. fowleri amebas in 5 mL of lake water (𝑛=3).

ATCC no.	Species	Average ± SD%
30467	Naegleria lovaniensis	1.5±1.1%
30811	Naegleria lovaniensis	14±3.5%
PRA-166	Naegleria dunnebackei	2.8±0.6%
PRA-153	Naegleria italica	9.0±2.8%
30958	Naegleria australiensis	2.7±2.5%
30544	Naegleria clarki	8.5±3.4%
30877	Naegleria gruberi	3.4±0.7%
30900	Naegleria jadini	1.5±0.6%
30965	Vahlkampfia inornata	1.3±0.7%
30298	Vahlkampfia lobospinosa	14±3.3%
30703	Tetramitus jugosus	0.6±0.3%
50171	Echinamoeba exundans	3.9±0.9%
50237	Hartmannella vermiformis	1.0±0.2%