Mast Cell Subsets and Their Functional Modulation by the Acanthocheilonema viteae Product ES-62
Chemokine and cytokine release by mast cell subsets. Mucosal-type BMMC (a), (c) & (e) and CTMC (b), (d) & (f) were sensitised with murine anti-DNP IgE (0.5 μg/mL) in the presence and absence of ES-62 (2 μg/mL) overnight. Cells were then stimulated with DNP (0.5 μg/mL) to induce FcεRI cross-linking (XL) or LPS (0.5 μg/mL) for 24 h at 37°C and MCP-1 (a) & (b), IL-6 (c) & (d), and IL-13 (e) & (f) release measured by ELISA. The data presented are single experiments representative of at least 2 independent experiments apart from the IL-13 release from CTMC, which could only be detected in a single experiment.