Investigating the Chaperone Properties of a Novel Heat Shock Protein, Hsp70.c, from Trypanosoma brucei
Figure 6
TbHsp70.c expression is enhanced under heat stress. Detection of TbHsp70.c expression levels in a whole cell lysate prepared from Lister 927 T. b. brucei v221 cells in the absence (37°C) and presence (42°C) of heat stress by 10% SDS-PAGE ( cells/lane); western analysis revealed that TbHsp70.c is heat inducible. Upper panel: Lanes 1 - cells incubated for 1 hour at 37°C; 2 – cells incubated for 1 hour at 42°C; 3 – purified recombinant TbHsp70.c in E. coli BB1994 [pQE80-TbHsp70.c]. Lower panel: Lanes 1–3 - detection of target protein by western analysis using anti-TbHsp70.c antibody, showing increased expression levels of TbHsp70.c under heat stress. The experiment was performed in triplicate using three different whole cell lysates and the figure represents the findings of a typical experiment.
