Novel Arsenic Nanoparticles Are More Effective and Less Toxic than As (III) to Inhibit Extracellular and Intracellular Proliferation of Leishmania donovani

Figure 1

Cytotoxicity assay of Leishmania promastigotes by As-NPs and As (III). (a) Determination of IC₅₀ value from the plot of log of promastigote cell numbers obtained after 24 hours of incubation with indicated concentrations of As-NPs. The error bars indicate SEM. (b) (i) The percentage of cell survival of Leishmania promastigotes treated with 0.1 μM (■), 1.0 μM (▲), and 2.0 μM (×) As-NPs for 24, 48, and 72 hours and those left untreated (◆) for the same time period was measured by MTT assay as mentioned in Section 2. The error bars indicate SEM. (b) (ii) The percentage of cell survival of Leishmania promastigotes treated with 0.1 μM (red line), 1.0 μM (blue line), 2.0 μM (green line), 10.0 μM (□), 20.0 μM (●), and 30.0 μM (△) As (III) for 24, 48, and 72 hours and those left untreated (◆) for the same time period was measured by MTT assay as mentioned in Section 2. The error bars indicate SEM.

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