Novel Arsenic Nanoparticles Are More Effective and Less Toxic than As (III) to Inhibit Extracellular and Intracellular Proliferation of Leishmania donovani

Figure 5

Effect of As-NPs on macrophages. (a) RAW cells were incubated without and with As-NPs (2 μM) for 24 and 48 hours at 37^°C as indicated, following which, cells were stained with DAPI. Phase and DAPI images for each set are shown. These images are representative of several images obtained from multiple independent experiments. (b) The number of the viable cells in control (0) and sets treated with indicated concentrations of As-NPs was plotted. The number of the viable cells was calculated as mentioned in Section 2. In a separate experiment, a standard curve was generated to determine the equivalence of the cell numbers and the O.D. values. The number of viable cells in the untreated control set (~4000) is less than that which was seeded initially (5000). Each column with error bar represents average ± SD, . denotes values (<0.05) of statistical significance.

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