Research Article

Evaluation of an Immunoassay-Based Algorithm for Screening and Identification of Giardia and Cryptosporidium Antigens in Human Faecal Specimens from Saudi Arabia

Table 4

Diagnostic tests’ results on 250 random stool samples.

Test resultMicroscopyThe algorithmaThe reference PCRsb

Cryptosporidium-positive132426
Giardia-positive334242
Crypto /Giardia-negative204184182
Total250250250

The algorithm picked 20 positive samples more than the staining methods adopted at the Microbiology Laboratory at King Faisal Hospital. These samples were also positive for protozoan DNA by the matching reference PCR assay. bTwo Cryptosporidium DNA positive samples were diagnosed by PCR and missed by the algorithm. cCrypto stands for Cryptosporidium.