Research Article
Histochemical Study of the Progenetic Trematode Alloglossidium renale
Table 1
Stains and staining specificity.
| Stain | Staining specificity | Controls: positive (P) and negative (N) |
| Alcian blue (pH 1.0) AB1 | Strongly acidic (sulfated) mucosubstances stain blue | Fish intestinal submucosa (P) |
| Alcian blue (pH 2.5) AB2.5 | Weakly acidic (carboxylated) mucosubstances stain blue | Fish intestinal submucosa (P) Shrimp hepatopancreas (P) |
| Ferro-ferricyanide FF | Melanin and tyrosine stain dark green/blue | Vitellarium (P) |
| Feulgen nuclear reaction FL | DNA stains red | Shrimp antennal gland nuclei (P) Schiff reagent without HCL treatment (N) |
| Harris hematoxylin HH | Nuclei stain dark purple and cytoplasm light brown | Shrimp antennal gland cells (P) |
| Ninhydrin Schiff NS | Amino acids stain pink or red | Shrimp muscle (P) Schiff reagent without ninhydrin treatment (N) |
| Osmium OS | Lipids stain black or olive | Oil droplets in cytoplasm (P) |
| Periodic acid-Schiff PAS | Neutral carbohydrates stain red | Fish intestinal goblet cells (P) Schiff reagent without PA (N) |
| Toluidine blue TB | Nuclei stain dark blue, acidic mucin and cytoplasm stain light blue, pink, or violet | Shrimp muscle and antennal gland nuclei (P) |
| Ward’s protein stain WPS | Amino acids stain blue | Shrimp muscle (P) |
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