Journal of Parasitology Research / 2014 / Article / Tab 1

Research Article

Histochemical Study of the Progenetic Trematode Alloglossidium renale

Table 1

Stains and staining specificity.

StainStaining specificity Controls: positive (P) and negative (N)

Alcian blue (pH 1.0)
AB1
Strongly acidic (sulfated) mucosubstances stain blueFish intestinal submucosa (P)

Alcian blue (pH 2.5)
AB2.5
Weakly acidic (carboxylated) mucosubstances stain blueFish intestinal submucosa (P)
Shrimp hepatopancreas (P)

Ferro-ferricyanide
FF
Melanin and tyrosine stain dark green/blueVitellarium (P)

Feulgen nuclear reaction
FL
DNA stains redShrimp antennal gland nuclei (P)
Schiff reagent without HCL treatment (N)

Harris hematoxylin
HH
Nuclei stain dark purple and cytoplasm light brownShrimp antennal gland cells (P)

Ninhydrin Schiff
NS
Amino acids stain pink or redShrimp muscle (P)
Schiff reagent without ninhydrin treatment (N)

Osmium
OS
Lipids stain black or oliveOil droplets in cytoplasm (P)

Periodic acid-Schiff
PAS
Neutral carbohydrates stain redFish intestinal goblet cells (P)
Schiff reagent without PA (N)

Toluidine blue
TB
Nuclei stain dark blue, acidic mucin and cytoplasm stain light blue, pink, or violetShrimp muscle and antennal gland
nuclei (P)

Ward’s protein stain
WPS
Amino acids stain blueShrimp muscle (P)