Histochemical Study of the Progenetic Trematode Alloglossidium renale

Table 1

Stains and staining specificity.

Stain	Staining specificity	Controls: positive (P) and negative (N)
Alcian blue (pH 1.0); AB1	Strongly acidic (sulfated) mucosubstances stain blue	Fish intestinal submucosa (P)
Alcian blue (pH 2.5); AB2.5	Weakly acidic (carboxylated) mucosubstances stain blue	Fish intestinal submucosa (P); Shrimp hepatopancreas (P)
Ferro-ferricyanide; FF	Melanin and tyrosine stain dark green/blue	Vitellarium (P)
Feulgen nuclear reaction; FL	DNA stains red	Shrimp antennal gland nuclei (P); Schiff reagent without HCL treatment (N)
Harris hematoxylin; HH	Nuclei stain dark purple and cytoplasm light brown	Shrimp antennal gland cells (P)
Ninhydrin Schiff; NS	Amino acids stain pink or red	Shrimp muscle (P); Schiff reagent without ninhydrin treatment (N)
Osmium; OS	Lipids stain black or olive	Oil droplets in cytoplasm (P)
Periodic acid-Schiff; PAS	Neutral carbohydrates stain red	Fish intestinal goblet cells (P); Schiff reagent without PA (N)
Toluidine blue; TB	Nuclei stain dark blue, acidic mucin and cytoplasm stain light blue, pink, or violet	Shrimp muscle and antennal gland; nuclei (P)
Ward’s protein stain; WPS	Amino acids stain blue	Shrimp muscle (P)