Histochemical Study of the Progenetic Trematode Alloglossidium renale

Table 2

Alloglossidium renale adult worm in situ staining results.

Stain	Tegument	Parenchyma	Vitellarium	Intestinal lining	Ova
Alcian blue (pH 1.0)	+++ outer tegument	−	−	+	−
Alcian blue (pH 2.5)	+++ outer tegument	−	−	++	−
Ferro-ferricyanide	−	−	+++	−	+
Feulgen nuclear reaction	++ inner tegument	+	++	−	++
Harris hematoxylin	+ outer tegument; light brown cytoplasm; + inner tegument; dark purple nuclei	++ light brown cytoplasm; + dark purple nuclei	+++ dark purple nuclei	+ cytoplasm; ++ dark purple nuclei	+ cytoplasm; ++ dark purple nuclei
Ninhydrin-Schiff	++	+ light	+++	++	+++
Osmium	−	++ random oil droplets	+	++	−
Periodic acid-Schiff	+ outer tegument; ++ inner tegument	+	+/−	++	++
Toluidine blue	+ outer tegument light blue; ++ inner tegument dark blue (Nuclei)	+ purple nuclei; + pink acidic structures	+++ dark blue nuclei	+ dark blue nuclei; ++ light blue acidic structures	+++ dark blue nuclei
Ward’s protein stain	++	+	++/−	++	++