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Journal of Parasitology Research
Volume 2014, Article ID 272601, 7 pages
Research Article

MHC-DRB1/DQB1 Gene Polymorphism and Its Association with Resistance/Susceptibility to Cystic Echinococcosis in Chinese Merino Sheep

College of Animal Science and Technology, Shihezi University, Xinjiang, 832003, China

Received 13 June 2013; Revised 23 October 2013; Accepted 23 October 2013; Published 24 March 2014

Academic Editor: C. Genchi

Copyright © 2014 Hong Shen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Fig S1: Electrophoretic patterns of PCR products of exon 2 of Ovar-DRB1/DQB1 in Chinese Merino sheep.

Fig S1: 10µl of DRB1 exon 2 PCR product was digested with 5U of Hin1I in Chinese Merino sheep, and then Samples were resolved by 1.5% agarose gel electrophoresis.

Fig S2: Each 10µl of DRB1 exon 2 PCR product was digested with 5U SacII, MroxI, ScaI, NciI respectively, The concentration of agarose gel electrophoresis is at2.5%.while concentration of agarose gel concentration of ScaI is 3%.

Fig S3: Comparison of MHC-DQB1 sequences of sheep.

TableS1: Conditions of restriction enzymes and examination for PCR products of the exon 2 of MHC-DRB1/ DQB1.

Table S2: The genotypes of PCR-RFLP in exon 2 of the Ovar-DRB1/gene.

  1. Supplementary Materials