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Journal of Parasitology Research
Volume 2014 (2014), Article ID 672018, 4 pages
Research Article

Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy

Department of Microbiology, Kasturba Medical College, Manipal University, Karnataka 576104, India

Received 18 February 2014; Revised 19 March 2014; Accepted 27 March 2014; Published 10 April 2014

Academic Editor: Bernard Marchand

Copyright © 2014 Vinay Khanna et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We have tried a new approach to routine stool microscopy by using a combination of methylene blue and glycerol in wet mount preparation of fresh faecal samples for the demonstration of medically important intestinal parasites. This combination was evaluated for finding differences in the details and clarity of morphology and internal structures of parasites under low- and high-power microscopy as compared to iodine and saline mount. It was further evaluated to estimate the time taken by methylene blue-glycerol mount to dry up as compared to iodine and saline wet mount.