Partial Purification of Integral Membrane Antigenic Proteins from Trypanosoma evansi That Display Immunological Cross-Reactivity with Trypanosoma vivax
Solubilization of the proteins contained in the fraction using SDS. The T. evansi fraction was homogenized using 4% SDS. Following centrifugation, the supernatant (SSDS) was separated from the SDS-washed pellet (PSDS). The concentration of SDS was reduced to 2% and the polypeptide composition of the SSDS and PSDS fractions was evaluated by SDS-PAGE. Shown is the Coomassie blue staining of the gel. M = protein molecular weight standards.