Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex

Figure 3

Comparison of relative activity of enzymes and expression of four genes encoding the enzymes trehalose-6-phosphate synthase (TPS), trehalose-6-phosphate phosphatase (TPP), glycogen synthase (GS), and glycogen phosphorylase (GP) using the normalization reference gene ryb between L3 and L4 larval stages of Anisakis simplex. Different letters (a, b, and c) above the bars represent significant differences between L3 (control) and L4 of A. simplex for gene expression and enzyme activity. Data from Łopieńska-Biernat et al. [25], for enzymes and mRNA of trehalose metabolism, for glycogen metabolism in L4 of A. simplex () (as control 100% activity of enzymes was 0.43 ± 0.02 U/mg for TPS and 0.34 ± 0.03 for TPP U/mg in L3).