Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex

Table 1

Nucleotide sequences of primers for PCR amplification of genes encoding the enzymes for glycogen metabolism.

Primers	Names	Primers (5′-3′)	Annealing temperature (°C)	Size of PCR product (bp)
For.	tps1s	GGGTCTTGGGAGATCAACA	50	268
Rev.	tps1as	TGCTGCTTTGGTGTCAACTC
For.	tpps	AGCGTTATTCAGTGGCTCGT	58	192
Rev.	tppas	CATGGCACTCTTTGTTGGTG
For.	gsFor	CAAYGTBGARTCVCTSAAAGGHC	55	485
Rev.	gsRev	ATRAARCADCCRAADCCDGAVAG
For.	gps	GATCGCCGTAAGCAGATCAGCGT	53	927
Rev.	gpas	CGGCATCCATACTTGCTTGATTTG