Research Article
Expression of Genes Encoding the Enzymes for Glycogen and Trehalose Metabolism in L3 and L4 Larvae of Anisakis simplex
Table 2
Primer sequences used for semiquantitative and real-time PCR.
| Gene | Accession number | Primers (5′-3′) | Size of PCR product (bp) |
| tps | /KJ560557 | For. GAAGTTACGTCAATCAATATGAGAAGG/ACTACGCATCACAAGCAACG | 163 | Rev. TTCAGGTCCACCCACCCATC/CGCTTGACGTATCAATGGAA |
| tpp | /KJ560558 | For. GTTTTCGCTTGTCTGCTCACG/TACGAAAGCATTCCAACGTG | 193 | Rev. TCGTTAGCGGCATTTCCTG/ATCGGATGATACGCTGCAAG |
| gs | JX173686 | For. GCCACCGATTTGCACACACAA | 173 | Rev. CAGCCACGCAAATTCCTCA |
| gp | JX941465 | For. GGATTCGCACACAACAATACTA | 193 | Rev. RCTGCGTCTTCTCGATCTCTTG |
| 18S | U81575 | For. ACCAGTAACGAAAGCGTGTG | 211 | Rev. GCTGTTGGAAGGAAGAACGA |
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This sequence data was used to design primers for real-time PCR.
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