Clinical Variation of Plasmodium falciparum eba-175, ama-1, and msp-3 Genotypes in Young Children Living in a Seasonally High Malaria Transmission Setting in Burkina Faso

Table 1

Sequences of the primers and PCR conditions.

	Sequences	PCR conditions
	eba-175
First; reaction	5′-CAAGAAGCAGTTCCTGAGGAA-3′ (forward); 5′-CTCAACATTCATATTAACAATTC-3′ (reverse)	D (94°C for 1 min), A (56°C for 1 min),; Ex (72° for 2 min), FEx (72°C for 3 min), Cy (29 cycles)
Second; reaction	5′-GAGGAAAACACTGAAATAGCACAC-3′ (forward); 5′ CAATTCCTCCAGACTGTTGAACAT-3 (reverse)	D (94°C for 1 min), A (56°C for 1 min),; Ex (72°C for 2 min), FEx (72°C for 3 min), Cy (24 cycles)
	ama-1
First; reaction	VM785/3: 5′-CCGGATCCCCTTTGAGTTTACATATATG-3′ (forward); VM990: 5′-AAA TTC TTT CTA GGG CAA AC-3′ (reverse)	D (95°C for 2 min 30 s), A (51°C for 30 s),; Ex (68°C for 45 s), FEx (68°C for 5 min), Cy (30 cycles)
Second; reaction	VM815/3: 5′-GGA ACT CAA TAT AGA CTT CC-3′ (forward); VM990: 5′-AAA TTC TTT CTA GGG CAA AC-3′ (reverse)	D (95°C for 2 min 30 s), A (51°C for 30 s),; Ex (68°C for 45 s), FEx (68°C for 5 min), Cy (35 cycles)
	msp-3
First PCR; reaction	159F: 5′-ATGTTGCTAGTAAAGAAATTG-3′ (forward); 745R: 5′-CATAACTAGAAGCTTCTTTTGC-3′ (reverse)	D (94°C for 30 s), A (54°C for 30 s),; Ex (68°C for 2 min), FEx (68°C for 5 min), Cy (30 cycles)
Second PCR; reaction	188F: 5′-ATAATCTTAACTTAAGAAATGC-3′ (forward); 745R: 5′-CATAACTAGAAGCTTCTTTTGC-3′ (reverse)	D (94°C for 30 s), A (54°C for 30 s),; Ex (68°C for 2 min), FEx (68°C for 5 min), Cy (40 cycles)

D: denaturation; A: annealing; Ex: extension: FEx: final extension; Cy: number of cycles.