Development of a Loop Mediated Isothermal Amplification for Diagnosis of Ascaris lumbricoides in Fecal Samples

Figure 2

(a) Shows the sensitivity of LAMP visualized by 2% agarose gel electrophoresis. Lanes 1, 2, 3, 4, 5, and 6 are DNA extracted from 1, 5, 10, 15, 20, and 25 eggs, respectively. Lane M is the 100 bp molecular marker and N is the negative control. (b) LAMP visual detection for color change using the SYBR Green dye. Tubes 1, 2, 3, 4, 5, and 6 represent DNA extracted from 1, 5, 10, 15, 20, and 25 eggs, respectively, through 6. N is negative control.

(a)

(b)