Development of a Loop Mediated Isothermal Amplification for Diagnosis of Ascaris lumbricoides in Fecal Samples

Figure 3

(a) Specificity of LAMP assay by gel electrophoresis. Lane 1, A. lumbricoides adult worm; lane 2, Ascaris ova DNA; lane 3, hookworm ova DNA; lane 4, S. mansoni ova DNA; lane 5, T. trichiura ova DNA; lane N, negative control; and M = 100 bp molecular ruler (Thermo Scientific). (b) Visual detection by color change using the SYBR Green dye. Tube 1, A. lumbricoides adult worm; tube 2, Ascaris ova DNA; tube 3, hookworm ova DNA; tube 4, S. mansoni ova DNA; tube 5, T. trichiura ova DNA; and lane N, negative control.

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