Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis

Figure 5

Binding of the SrTrx-lp (a) and TsTrx-lp (b) to different cell types. 2 × 10⁵ cells were incubated at 37°C for 30 min with Alexa Flour®-labeled SrTrx-lp or TsTrx-lp. Here, peripheral blood cells (monocytes, granulocytes, and lymphocytes) as well as cell culture cells (Caco-2 cells, THP-1 cells, and THP-1-derived DCs) were tested with 0.4 μg (purple (a), red (b) line) and 0.6 μg (blue (a), green (b) line) of labeled protein determining the median fluorescent intensity (MFI). The intensity of surface fluorescence (FI, -axis) is plotted against cell counts. (The counts in the figures represent the median fluorescence index values.) Representative results of five independent experiments are shown.

(a)

(b)