Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis

Figure 2

L. major pXG-mCherry12 parasites stably expressing the red fluorescent protein, upholding the same growth rate as the WT strain. (a) Fluorescent detection of red fluorescent parasites by confocal microscopy. Top lane, single parasite acquisition (100x objective). Bottom lane, more parasites per field (40x objective). Microscopy images were acquired with a Nikon Eclipse E800 confocal microscope. (b) Wild type strain and pXG-mCherry12 transfected parasites growth curve. Growth was measured during 6 days for WT (filled circle “●”) and pXG-mCherry12 (filled diamond “◆”) transfected parasites cultures. Data represents the mean (± SD) of three independent experiments.

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