Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis

Figure 4

pXG-mCherry12 transfected parasites preserve their fluorescence during in vitro and after in vivo infections. (a) Murine peritoneal macrophages infected with red fluorescent parasites. Solid white arrows indicate fluorescent amastigotes. Microscopy images were acquired with a Nikon Eclipse E800 confocal microscope. (b) Fluorescence emitted from pXG-mCherry12 promastigotes recovered after in vivo infection. 96-well plates were read with a FLUOstar Optima plate reader using 570-nm excitation and 620-nm absorbance filters.

(a)

(b)