Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis

Table 2

Drug activity profile of L. major cell lines. Promastigotes were grown, as described in Materials and Methods, for 48 and 72 hours at 26°C in the presence of increasing drug concentrations. The 50% effective concentrations (EC₅₀) were measured using an MTT-based assay and a FLUOstar Optima fluorimeter for WT and pXG-mCherry12 parasites. Results are expressed as mean (± standard error [SEM]) from three independent experiments.

Parasite	EC50 (μM) mean ± SEM
	Amphotericin B (48 h)		Amphotericin B (72 h)		Miltefosine (48 h)		Miltefosine (72 h)
	MTT	Fluorescence	MTT	Fluorescence	MTT	Fluorescence	MTT	Fluorescence
WT	0.11 ± 0.014		0.13 ± 0.031		5.075 ± 0.52		6.97 ± 0.71
pXG-mCherry12	0.12 ± 0.0040	0.14 ± 0.012	0.15 ± 0.031	0.13 ± 0.021	5.16 ± 1.21	5.58 ± 1.022	5.48 ± 1.063	6.32 ± 0.63