Identification, Characterization, and Structure of Tm16 from Trichuris muris

Figure 3

Production and localization of Tm16. (a) SDS-PAGE of ~2 μg rTm16 expressed in P. pastoris X-33 after being induced with 5% methanol for 24–72 hours and IMAC purification. (b) Western blot showing the native Tm16 is in the T. muris ES products; rTm16 is slightly larger than the protein in the ES because it has a hexahistidine tag. The rTm14-3-3 (50 ng) was used as a negative control.

(a)

(b)