Journal of Parasitology Research

Research Article

Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits

Table 2

Samples with discordant results obtained from different test kits.


Sample number	Mic.	Giardia				Cryptosporidium
Sample number	Mic.	Kit A	Kit B	Kit C	Kit D	Kit A	Kit B	Kit C	Kit D

6	Giardia	0/0	0/0	0/0	0/0	0/0	0/0	0/0	0/0
7	Giardia	+/+	0/0	+/0	+/+	0/0	0/0	0/0	0/0
8	Giardia	+/+	+/+	+/+	0/0	0/0	0/0	0/0	+/0
9	Giardia	+/+	+/+	+/+	+/0	0/0	0/0	0/0	0/0
12	Giardia	+/+	+/0	+/0	0/0	0/0	0/0	0/0	0/0
15	Crypto	0/0	0/0	0/0	0/0	+/+	0/0	+/+	+/+
18	Crypto	0/0	0/0	0/0	0/0	+/+	+/+	+/0	+/+
23	Crypto	0/0	0/0	0/0	0/0	0/0	0/0	0/0	0/0
24	Crypto	0/0	0/0	0/0	0/0	+/+	+/+	0/0	+/+
32	undet	+/+	0/0	0/0	0/0	0/0	0/0	0/0	0/0
33	undet	+/0	0/0	0/0	0/0	0/0	0/0	0/0	0/0
34	undet	+/+	+/+	+/+	+/+	0/0	0/0	0/0	0/0
35	undet	+/+	+/+	+/+	+/+	0/0	0/0	0/0	0/0
42	undet	0/0	0/0	0/0	0/0	+/+	+/+	+/+	+/+
49	undet	+/+	+/+	+/+	+/0	0/0	0/0	0/0	0/0

Total positive		9	6	7	5	4	3	3	5

Mic = microscopy; undet = undetected; + = positive; 0 = negative. Results from duplicate tests are shown as +/+, +/0, and 0/0. Discordant results are in bold. Kit A = in-house RT-PCR assays, kit B = RIDA GENE Parasitic Stool Panel (PG1705), kit C = LightMix Modular Gastroenteritis Assays, and kit D = BD MAX Enteric Parasite Panel.